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Chapter 26 Instrumental Analysis
- Michael Clugston, Michael Clugstonformerly Tonbridge School
- Malcolm StewartMalcolm StewartUniversity of Oxford
- , and Fabrice BirembautFabrice BirembautCaen, France
Abstract
This chapter expounds on the types of instrumental analysis used in chemical laboratories, especially those working with organic compounds. It also notes the fragmentation patterns that characterize a molecule. Mass spectrometry is utilized to search for a compound's molecular formula, while infrared spectroscopy involves the absorption of radiation in the electromagnetic spectrum's infrared region. The energy absorbed makes the bonds vibrate more energetically. Nuclear magnetic resonance (NMR) spectroscopy calculates nuclei's protons and neutrons that possess nuclear spins. The chapter also explains the process of spin-spin splitting or coupling in the NMR spectra as it depends on the number of adjacent protons.