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Molecular Biology of RNA

Molecular Biology of RNA (Second Edition)

David Elliott and Michael Ladomery
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date: 18 July 2024

p. 20510. Messenger RNA localizationlocked

p. 20510. Messenger RNA localizationlocked

  • David ElliottDavid ElliottInstitute of Genetic Medicine, Newcastle University
  •  and Michael LadomeryMichael LadomeryFaculty of Health and Applied Science, University of the West of England

Abstract

This chapter is focused on the localization of mRNAs1-6, including the localization of microRNAs in neurons. It describes fluorescence in situ hybridization (FISH). Fluorescence uses in situ hybridization wherein a complementary antisense nucleic acid probe is labelled typically with a fluorescent probe. The chapter also highlights how FISH techniques have been greatly improved, to the extent that it is now possible to examine the localization of thousands of mRNAs. The chapter discusses the importance of mRNA localization, which is powerfully illustrated by a genome-wide analysis by Eric Lecuyer and colleagues of mRNA localization during Drosophila embryogenesis. It looks at the machinery of mRNA localization, including the role of the cytoskeleton, RNA zipcodes, and the influence of the nuclear history of a transcript on its localization.

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