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Cover Biophysical Techniques

Introduction to absorption and emission spectra  

Introduction The development and application of spectroscopy has had an enormous influence on our understanding of the world; from microscopic atomic structure (nm) to the macroscopic (light year scale!) universe. William Wollaston discovered the existence of dark lines in the solar spectrum in 1801. A...

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Cover Biophysical Techniques

Thermodynamics  

The aim of this tutorial is to introduce some key concepts about thermodynamics, which is the study of the energetics of a system. Life depends on the redistribution of energy. The study of thermal energy (heat) has a fascinating history; the first and second...

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Cover Biophysical Techniques

Analytical centrifugation  

Introduction Hand-driven centrifuges were used in the middle ages to separate milk products. Today, a variety of preparative centrifuges, ranging from small desktop to large scale devices, are in routine use in almost all molecular biology laboratories. We focus here on the analytical ultracentrifuge (AUC),...